PNA-Encoded Protease Substrate Microarrays
نویسندگان
چکیده
منابع مشابه
Selective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays
PNA probes for the specific detection of DNA from olive oil samples by microarray technology were developed. The presence of as low as 5% refined hazelnut (Corylus avellana) oil in extra-virgin olive oil (Olea europaea L.) could be detected by using a PNA microarray. A set of two single nucleotide polymorphisms (SNPs) from the Actin gene of Olive was chosen as a model for evaluating the ability...
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We have used time-of-flight secondary ion mass spectrometry (TOF-SIMS) to examine different immobilization processes of peptide nucleic acid (PNA) and their influence on the hybridization efficiency of unlabeled DNA fragments to complementary PNA. Two different approaches have been used to immobilize PNA onto Au surfaces. One method was to immobilize thiolated PNA in a single step reaction to t...
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Several strategies have been developed for the production of peptide nucleic acid (PNA) microarrays by parallel probe synthesis and selective coupling of full-length molecules. Such microarrays were used for direct detection of the hybridisation of unlabelled DNA by time-of-flight secondary ion mass spectrometry. PNAs were synthesised by an automated process on filter-bottom microtitre plates. ...
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A novel microarray-based proteolytic profiling assay enabled the rapid determination of protease substrate specificities with minimal sample and enzyme usage. A 722-member library of fluorogenic protease substrates of the general format Ac-Ala-X-X-(Arg/Lys)-coumarin was synthesized and microarrayed, along with fluorescent calibration standards, in glycerol nanodroplets on microscope slides. The...
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A "one bead two compound" approach to the synthesis of encoded cyclic peptoid libraries is reported.
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2004
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2004.07.015